lc3 5f10 cleaved caspase 3 asp175 cell signaling technology  (Cell Signaling Technology Inc)

Journal: Molecules

Article Title: Store-Operated Calcium Entry Inhibition and Plasma Membrane Calcium Pump Upregulation Contribute to the Maintenance of Resting Cytosolic Calcium Concentration in A1-like Astrocytes

doi: 10.3390/molecules28145363

Figure Lengend Snippet: Expression of PMCA in homogenates from astrocyte (U251 cell line) cultures. ( A ) Twenty-microgram homogenates from U251 cells untreated (C) or treated (A1-like astrocytes) with TNF-α (30 ng/mL), IL-1α (3 ng/mL) and C1q (400 ng/mL), were loaded onto a 10% SDS-polyacrylamide gel, electrotransferred to a PVDF membrane, and immunostained with the PMCA antibody 5F10. The anti-GAPDH antibody was used as a protein loading control. ( B ) Quantification of PMCA protein levels in Control and A1-like astrocytes relative to GAPDH is shown as mean ± SEM values, in arbitrary units. Statistical analysis was carried out using the Student’s t -test. (*) p < 0.05 with respect to each Control.

Article Snippet: The primary anti-PMCA antibody (clone 5F10) was purchased from Invitrogen.

Techniques: Expressing